1
Nce explained during this work is deposited in GenBank/ EMBLNce explained in this do the job has been deposited in GenBank/ EMBL/DDBJ beneath the accession No: AB971668.Semi-quantitative RT-PCRTotal RNA was isolated making use of TRIZOL (Invitrogen, Carlsbad, CA) and reverse-transcribed using oligo (dT)18 primers and M-MLV reverse transcriptase (Promega, Madison, WI) in accordance to the man
1
Nce explained during this work is deposited in GenBank/ EMBLNce explained in this do the job has been deposited in GenBank/ EMBL/DDBJ beneath the accession No: AB971668.Semi-quantitative RT-PCRTotal RNA was isolated making use of TRIZOL (Invitrogen, Carlsbad, CA) and reverse-transcribed using oligo (dT)18 primers and M-MLV reverse transcriptase (Promega, Madison, WI) in accordance to the man
1
Initial PCR was performed by making use of exon 4a-specific forward primer (4a-F, 5'ATTCCATTGCCTTTCCACAG-3') and 3' RACE outer reverse primer (5'-GCGAGCACAGA ATTAATACGACT-3'). Nested PCR was done through the use of the first PCR item like a template while using the nested Ex4a(+)WT1 cDNA-specific forward primer (4a-F2, 5'-GCAAAGCTCT CTGTG ACATTA-3') as well as nested adaptor internal primer (5'-CG