1
Initial PCR was performed by making use of exon 4a-specific forward primer (4a-F, 5'ATTCCATTGCCTTTCCACAG-3') and 3' RACE outer reverse primer (5'-GCGAGCACAGA ATTAATACGACT-3'). Nested PCR was done through the use of the first PCR item like a template while using the nested Ex4a(+)WT1 cDNA-specific forward primer (4a-F2, 5'-GCAAAGCTCT CTGTG ACATTA-3') as well as nested adaptor internal primer (5'-CG