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Initially PCR was carried out by making use of exon 4a-specific ahead primer (4a-F, 5'ATTCCATTGCCTTTCCACAG-3') and 3' RACE outer reverse primer (5'-GCGAGCACAGA ATTAATACGACT-3'). Nested PCR was carried out by making use of the main PCR merchandise being a template with all the nested Ex4a(+)WT1 cDNA-specific forward primer (4a-F2, 5'-GCAAAGCTCT CTGTG ACATTA-3') along with the nested adaptor inner p